To determine the longitudinal effects of tumor growth on the health of host flies, we conducted tumor transplantation studies of NICD-TZ tumors (Wang et al, 2021), which were induced in larval salivary gland imaginal ring transition zone (TZ) by over-expressing NICD with the Act-Gal4; Gal80Ʌts system (Actts) at 29 °C. This evidence concerns the gene LGALS4 and neoplasm.