To further elucidate the potential of PRPS1 to act as a substrate of AMPKα1, the expression levels of AMPKα1, p-AMPKα1(T172), PRPS1 and p-PRPS1(S180) were determined in melanoma cells that were glucose deprived or treated with AIACR, an AMPK activator that can markedly increase the level of phosphorylated AMPK. This evidence concerns the gene PRKAA1 and melanoma.