THBS1 and neoplasm: After 24 h, neurons co-cultured with SB28-TSP1-WT tumor cells exhibited a modest but consistent trend toward an increase in activity-related parameters, including increases in the total number of calcium events per neuron, as well as amplitude and kinetics (rise and fall times) of calcium currents, compared to the baseline neuron only condition (Fig. 3c, d, Supplementary Fig. 12 and Supplementary Movies 1–2).