THBS1 and glioma: By screening three publicly available murine RNA-seq datasets (in vitro SB28 and GL261 syngeneic glioma cell lines, and bulk normal mouse brain), we found that endogenous gene expression of TSP1 (Thbs1) was significantly higher in SB28 compared to the other two datasets (vs. GL261: log2fold change [FC] = 4.50, adjusted p = 7.4 × 10−60; vs. normal mouse brain, log2FC = 9.54, adjusted p < 1 × 10−300) (Fig. 3a).