We first confirmed that the S protein underwent S-palmitoylation via thioester bonds in the PEDV-infected African green monkey kidney epithelial Vero cells (Fig. 7A), whereas non-cytotoxic 2-BP treatment observably decreased the palmitoylation and protein levels of S protein during infection (Fig. 7B; Fig. S1B). This evidence concerns the gene PROS1 and infection.