In order to establish a more severe ischemic stroke model embracing subcortical regions including the striatum, we injected endothelin‐1 (ET‐1, 1–31), a blood vessel constricting factor, to the vicinity of the rat middle cerebral artery to establish a MCAO (middle cerebral artery occlusion)‐like ischemic stroke model as previously described [33]. Here, EDN1 is linked to ischemic stroke.