This study found significant upregulation of SPC25 in PCa. SPC25 knockdown inhibited proliferation, increased G2/M-phase cells, and promoted apoptosis. Bioinformatics analysis showed SPC25 regulates proliferation, apoptosis, invasion, and TGF-β pathways. TCGA data revealed SPC25 upregulation in advanced PCa, and lower SPC25 expression was linked to better survival. Here, TGFB1 is linked to posterior cortical atrophy.