Label-free examples includes breast cancer cells MDA-MB-231 imaged by phase contrast at 10X (Fig. 2a) and bright-field at 40X (Fig. 2b); differential interference contrast (DIC) images of MDA-MB-231 at 63X (Fig. 2c.i) and S. cerevisiae (Fig. 2c.ii); human fibroblast Hs27 imaged by interference reflection microscopy at 40X (Fig. 2d); epifluorescence images of GFP-labeled A549 cells (Fig. 2e.i and ii) at 40X (previously analyzed as live cell imagery in Robitaille et al. and Christodoulides et al.17,28,29), F-actin and vinculin-labeled MDA-MB-231 cells (Fig. 2e.iii and iv) at 63X. This evidence concerns the gene VCL and breast cancer.