Combining these data, 125I particle radiotherapy effectively enhanced the expression of ROS, USP7, and P53 through the HSF1/PU.1/SYK signaling pathway, and inhibited the growth and proliferation of cervical cancer C33A cells, suggesting that the HSF1/PU.1/SYK signaling pathway may be the key mechanism of action of 125I particle radiotherapy. Here, HSF1 is linked to cervical cancer.