As one of the main transcriptional regulators of the heat shock response (HSR), heat shock factor 1 (HSF1) plays an important role when cells and organs are exposed to heat, ischemia, hypoxia, inflammation, and other adverse stress factors.14–17 This study endeavors to elucidate the cytotoxic effects of 125I particle radiotherapy on cervical cancer cells and the underlying mechanisms, particularly through the upregulation of the HSF1/PU.1/SYK signaling pathway, which subsequently enhances the ROS/USP7/p53-mediated apoptotic response and inhibits cervical cancer progression. This evidence concerns the gene SPI1 and cervical cancer.