AR and neoplasm: Moreover, to further demonstrate that the stemness impairment produced by ENZ is due to the direct inhibition of AR and not to possible off-targets effects, we also evaluated GBM stemness after the direct silencing of AR and we found an impaired tumor-sphere forming ability, decreased spheroid frequency and reduced stem markers expression as observed after miRNA signature overexpression or WT1 and FOXA1 silencing (Fig. 4F, G).