Moreover, to further demonstrate that the stemness impairment produced by ENZ is due to the direct inhibition of AR and not to possible off-targets effects, we also evaluated GBM stemness after the direct silencing of AR and we found an impaired tumor-sphere forming ability, decreased spheroid frequency and reduced stem markers expression as observed after miRNA signature overexpression or WT1 and FOXA1 silencing (Fig. 4F, G). Here, AR is linked to glioblastoma.