Among these TFs, SOX6, SOX15, CEBPG, KLF13, NFAT5, and SMAD1 were experimentally validated for their ability to affect the migration and invasion of breast cancer cells, the transwell assay results showed that knockdown of these TFs, respectively, significantly decreased the migration (Figures S13b and S14b, Supporting Information) and invasion (Figures S13c and S14c, Supporting Information) of both cancer cells. The gene discussed is SOX6; the disease is cancer.