Our study using metaTF on scRNA‐seq breast cancer datasets found that CD8+ TEM cells could be divided into two clusters based on TF activity, with the ADRB2+ CD8+ TEM subset showing higher expression of activation and proliferation markers and genes related to immune responses, and validation experiments demonstrated that ADRB2+ CD8+ TEM cells respond to neuro‐related signals by activating key transcription factors such as BCL6, TBX21, and ATF3, along with their downstream targets. This evidence concerns the gene CD8A and breast cancer.