To assess the role of TMEM150C/DRAM4 and/or TMEM150A/DRAM5 in modulating autophagy, we overexpressed either TMEM150C/DRAM4 or TMEM150A/DRAM5 in a human osteosarcoma cell line (Saos2), which expresses an average level of TMEM150C/DRAM4 and TMEM150A/DRAM5 mRNA. Here, TMEM150C is linked to osteosarcoma.