In this study, we analysed the expression level of HSPH1 in NSCLC and its value for diagnosis and prognosis by bioinformatics methods, explored the potential mechanism of HSPH1 in NSCLC by pathway enrichment analysis and immune infiltration analysis, and further validated the bioinformatics results by experiments and collection of clinical samples, with the aim of providing new ideas for early diagnosis, targeted intervention and We hope to provide new ideas for early diagnosis, targeted intervention and prognostic evaluation of NSCLC. Here, HSPH1 is linked to non-small cell lung carcinoma.