To probe whether the SLP-specific immune response was the key to efficient anti-tumor effects, the splenocytes of this mouse were re-stimulated with SLP and the secretion of IFN-γ and Granzyme B were detected through ICCS, and finally robust production of Granzyme B in CD8+ T cells was monitored, whereas IFN-γ-secreting CD8+ T cells were rarely noticed (in supplementary data). The gene discussed is CD8A; the disease is neoplasm.