Gene set enrichment analysis (GSEA) also revealed a significant downregulation of the hypoxia pathway and HIF-1α target genes upon ASH1L depletion (Fig. 3a–d), particularly those involved in cancer cell invasion (Snail, TGFB, and MET), extracellular matrix remodeling (MMPs and PLAU), and angiogenesis (VEGFs). Here, ASH1L is linked to cancer.