Gene set enrichment analysis (GSEA) also revealed a significant downregulation of the hypoxia pathway and HIF-1α target genes upon ASH1L depletion (Fig. 3a–d), particularly those involved in cancer cell invasion (Snail, TGFB, and MET), extracellular matrix remodeling (MMPs and PLAU), and angiogenesis (VEGFs). This evidence concerns the gene MET and cancer.