Since a prognostic impact for CD276 was found and the CD4 and CD8 gene expression were associated with HIV status, 48 BC specimens, containing 24 cases of WLWH (8 WLWH–VS, 16 WLWH–IS) and 24 cases of HIV negative patients were subjected to MSI using two 8-plex panels including CD3, CD8, FoxP3, IDO-1, PD-1, LAG-3, CTLA4, and CD69, as well as panCK, CD3, pStat1, ISG15, HLA-I heavy chain (HC), HLA-G, TAP1, and CXCR4. This evidence concerns the gene ISG15 and breast cancer.