The protein expression of Western blotting showed that the nutraceutical complex has the protective effect of NAFLD, which is specifically manifested as up-regulating the relative expression of TBC1D1 protein and down-regulating the relative expression of ACACA, ACSL1 and FBP2 protein (Fig 4 and S5 Table in S1 File). This evidence concerns the gene TBC1D1 and metabolic dysfunction-associated steatotic liver disease.