Analogous to the assessment of Xkr8 in cell intrinsic oncogenic features, TMEM16F knockout cells also did not show defects in tumor cell proliferation (Fig.4.E.) or tumor-sphere formation in Matrigel, as shown by tumor-sphere size (Fig. 4.F.) and tumor-sphere count (Fig 4.G.), or defects in the immediate Gas6-maediated activation of Akt (SFig 2B). The gene discussed is AKT1; the disease is neoplasm.