To assess the effects of TMEM16F knockout on tumor growth and the tumor microenvironment and compare effects to that of the Xkr8 knockout model described above (Fig. 3), EO771 WT cells or EO771 TMEM16F KO cells were transplanted orthotopically into the mammary gland fat pads of C57BL/6 WT mice. This evidence concerns the gene XKR8 and neoplasm.