IL31 and neoplasm: Multiplex bead-based ELISA (Luminex) analysis of cell culture supernatant from 4T1 Vector, 4T1 MerTK C8, and 4T1 MerTK C11 tumor cells revealed significantly increased release of the pro-inflammatory cytokines IL-1 alpha and IL-31, and chemokines MCP-1 (CCL2) and MCP-3 (CCL7) (Figure 2F).