Therefore, the study aimed to examine the cardio-protection impact of various spices in Winstar albino rat hearts following isoproterenol-induced myocardial damage utilizing anthropometric, histological, and Caspase-3 mRNA expression studies as well as to identify bioactive natural compounds of fourteen mixed spices through molecular docking, ADME, toxicity and finally molecular dynamics simulation approaches identified the lead compound as an alternative treatment for myocardial infarction. Here, CASP3 is linked to myocardial infarction.