To better assess mitochondrial activity, we next performed a MitoStress test, monitoring OCR after exposure of AML cells to the LDHA inhibitors, followed by injection of oligomycin, FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; an oxidative phosphorylation uncoupler) and AA + Rot (antimycin A and rotenone; respectively inhibiting Complex III and Complex I of the electron transport chain) into the culture medium. Here, LDHA is linked to acute myeloid leukemia.