To test whether the reduced NAD+/NADH ratio was responsible for cell death induction upon LDHA inhibition in AML cells, we attempted to re-balance the NAD+/NADH ratio by transducing NB4 cells with lentiviral constructs overexpressing a mitochondrial or cytosolic variant of the water-forming NADH oxidase (NOX) from Lactobacillus brevis (LbNOX) [24]. The gene discussed is LDHA; the disease is acute myeloid leukemia.