ANXA1, as a scaffold protein, highly expressed ANXA1 can recruit USP5 to stabilize GOT1, thereby promoting GOT1 mediated glutamine metabolism, further promoting GSH production and reducing ROS levels, reducing oxidative stress, and promoting ICC cell growth in vitro and in vivo (Fig. 8A). The gene discussed is GOT1; the disease is intrahepatic cholangiocarcinoma.