To validate the essentiality of the 12 target gene candidates using an orthogonal approach, we performed an arrayed competition-based growth proliferation assay using two doxycycline-inducible small hairpin RNAs (shRNA) targeting each transcript in NUP98::KDM5A AML cells stably expressing the reverse Tet-transactivator (rtTA3)10 (Fig. 5C and Table 1). Here, KDM5A is linked to acute myeloid leukemia.