As SB-T-101141 triggered cell death in an iron-dependent manner, we found that SB-T-101141 enhanced cellular iron and ferrous ion levels as well as the MDA and lipid ROS formation, which could be significantly neutralized by KHSRP depletion or DFOM in breast cancer cells, despite that knocking down KHSRP elevated lipid ROS (Fig. 7A–E), indicating the mediator effector of ferroptosis of KHSRP in SB-T-101141 cytotoxicity. Here, KHSRP is linked to breast cancer.