is associated with anxiety- and depression-like behaviors and abnormalities in neuronal cell morphology in Negr1 KO mice, we administrated a single strain (m3-2) of the Akkermansia isolates or PBS to Negr1 KO and WT mice every weekday for 4 wk (Supplementary Fig. S3A). The gene discussed is NEGR1; the disease is major depressive disorder.