In this study, the combination of prospective cell lineage analysis using the GlastCreER;Rosa26LSL-Sun1-GFP transgene before infection with GFAP-Pou5f1-mCherry vector, in combination with single-cell RNA-sequencing (scRNA-seq) analysis of infected cells, rules out any potentially confounding factors resulting from the GFAP minipromoter. Here, POU5F1 is linked to infection.