Conversely, a decreasing trend was observed in the use of TRBV5‐5, TRBV5‐8, TRBV6‐4, TRBV6‐5, and TRBV10‐1 genes in the HD, CBD, and CRC groups and of TRBV3‐1, TRBV4‐1, TRBV5‐6, TRBV5‐8, TRBV7‐4, TRBV10‐1, TRBV10‐2, TRBV12‐5, TRBV24‐1, TRBV27, and TRBV28 in the HD, BNC, and NSCLC groups. The gene discussed is TRBV27; the disease is colorectal carcinoma.