Using novel tools, including PhosL lectin immunofluorescence and N‐glycan MALDI mass spectrometry imaging (MALDI‐MSI), we find FUT8 regulates the expression of malignant core fucosylated N‐glycans in prostate cancer cells, and using both in vitro and in vivo models, we show FUT8 can promote prostate tumour growth and increase cell migration and invasion. Here, FUT8 is linked to prostate cancer.