Further examination of differentially expressed genes in renal cysts and SC transplants demonstrated significant downregulation of key meiotic proteins, including Sycp3 and Sycp1 (Fig. 3I), as well as defects in DSB-related proteins Rad51 and Spo11, which align with our previous findings (Figs. 2B and 3D). This evidence concerns the gene SPO11 and cystic kidney disease.