GFP-tagged EGFR in combination with Myc-tagged WT-USP8, USP8-P681Q variant, and the USP8-S718P variant as well as empty vector were transiently transfected into corticotroph tumor cells followed by immunoprecipitation with anti-GFP antibody linked agarose beads. The gene discussed is EGFR; the disease is neoplasm.