Given the migratory and proliferative effects of sgMT1E revealed through both tRNA and miRNA analysis, we validated this transcriptional phenotype in an in vitro wound-healing assay (Fig. 3k), which showed a significant increase in wound closure in sgMT1E knockdown cells compared to the control, providing functional evidence that MT1E inhibits cell migration and proliferation, further implicating its function as a tumor suppressor. This evidence concerns the gene MT1E and neoplasm.