Colony formation assays validated tumor suppressor function of sgSEMA6B, sgMT1E, sgS100A, and sgASAH2 from the Perturb-DBiT top ten enriched hits (Fig. 4a and Extended Data Fig. 6c).This was exemplified by the inclusion of four previously reported tumor suppressors (ARHGAP6, CREBZF, DHRS7C, and NR4A3)66–69, whose corresponding sgRNAs were abundantly detected by Perturb-DBiT. This evidence concerns the gene DHRS7C and neoplasm.