In this study, we used ten tumor differentiation grade-related genes for the first time to establish the prognostic signature including TNFAIP2, MAGEA3, CXCR4, COL1A1, FN1, VCAN, PXDN, COL5A1, MUC13, and RGS2 based on single-cell RNA-seq. This evidence concerns the gene COL5A1 and neoplasm.