Our transcriptomic data revealed additional genes of mucin MUCL1, MUC4, MUC15 and MUC17, endocytic UBR3, mitotic FRY, metabolic CASTOR1, IRS1, and SGK1, apoptotic NTRK1, metastatic SLC16A1, and autophagic CFLAR‐AS1, ATG13 and ULK1 that were mutated in our DTX‐resistant laryngeal cancer cells, which have not been identified to be associated with DTX resistance in other types of cancer before. Here, IRS1 is linked to laryngeal carcinoma.