MAPK3 and congenital secretory chloride diarrhea 1: Our key findings demonstrate that: (i) TFNAs ameliorate ANIT-induced hepatocyte injury in vitro (Figure 2); (ii) TFNAs play a protective role in the ANIT-induced CLD model, mitigating liver injury and restoring BA homeostasis (Figure 4); and (iii) TFNAs activate the Wnt/β-catenin signaling pathway and promote ERK1/2 phosphorylation, providing antioxidant effects both in vivo and in vitro (Figures 3 and 5).