MCCC2 and hepatocellular carcinoma: To test if the deacetylation of MCCC2 at K269 contributes to the acquisition of stem cell-like properties of HCC TICs, we reconstituted the expression of sgRNA-resistant wild type (WT), deacetylated mimetic mutants (K141R and K269R), and acetylated mimetic mutants (K141Q and K269Q) in Huh-7 and PLC/PRF/5 cell lines with endogenous MCCC2 knocked out using CRISPR/Cas9 technique (Figure 5A and B).