Our finding that SIRT4-mediated MCCC2-K269 deacetylation resulted in increased acetyl-CoA production which induced H3K27 acetylation and TIC properties prompted us to hypothesize that SIRT4-mediated MCCC2-K269 deacetylation prompted HCC TIC properties by modulating the pathway(s) involved in stemness regulation via H3K27ac. The gene discussed is MCCC2; the disease is hepatocellular carcinoma.