Histopathological evaluation via H&E staining confirmed MASLD-associated steatosis and fibrosis, while IHC revealed that TPPP3 and COL10A1 localized to fibrotic septa and peri-sinusoidal regions, with staining intensities strongly correlated with fibrosis severity (Figure 6D). The gene discussed is COL10A1; the disease is steatosis.