The predominant hypothesis was that MARCH8 reduces nSREBP1 by degrading the SREBP1 protein in the cytoplasm of HCC cells, thereby inhibiting the expression of ACC1 and FASN. To substantiate this hypothesis, a cytoplasmic and nuclear fractionation assay was conducted, which revealed that MARCH8 exerted a considerable effect on nSREBP1 expression. The gene discussed is SREBF1; the disease is hepatocellular carcinoma.