The study further investigated whether MARCH8 inhibits FA synthesis and HCC cell progression by suppressing the expression of ACC1 and FASN. To establish stable cell lines, ACC1 and FASN lentiviruses were transfected into Huh7 cell lines that overexpress - MARCH8 expression (Supplementary Fig. 3I, K, M). The gene discussed is FASN; the disease is hepatocellular carcinoma.