SREBF1 and hepatocellular carcinoma: The findings suggest that the regulatory effect of MARCH8 on lipids in HCC cells was predominantly dependent on SREBP1. Next, colony formation assays were performed, and found that the cells showed reduced and enhanced proliferative capacities after the overexpression and knockdown of MARCH8. However, simultaneously, the co-overexpression or co-knockdown of MARCH8 and SREBP1 did not yield a significant variation in cell proliferation compared to the control group (Fig. 8I–L).