To systematically observe the sequential changes of ferroptosis after cerebral ischemia from the acute phase (D0 and D3) to the chronic phase (D14 and D28), we examined alterations in the expression of ferroptosis‐associated proteins [Glutathione Peroxidase 4 (GPX4), Long‐chain acyl‐CoA synthetase 4 (ACSL4), and Transferrin receptor (TfR)], within the peri‐infarct cortex and hippocampus regions using a cerebral ischemia (MCAO) model (Figure 1A). This evidence concerns the gene TFRC and brain ischemia.