CSF2 and central nervous system cancer: When we analyzed the cytokine levels in the supernatants obtained from the co-culture of SB28 glioma and NKT cells in the presence or absence of the CAR-mediated signaling, CAR-NKT cells co-cultured with SB28EGFRvIII cells showed a CAR-EGFRvIII-specific, significant increase in the secretion of IL-4, IL-17, and GM-CSF (Figure 3B).