ROC curve analysis demonstrated that CTU2 has high diagnostic accuracy (AUC > 0.8) for eight cancer types, including READ, LUSC, LUAD, kidney renal papillary cell carcinoma (KIRP), KIRC, kidney chromophobe (KICH), COAD, and bladder urothelial carcinoma (BLCA) (Figure 3C). This evidence concerns the gene CTU2 and bladder transitional cell carcinoma.