To this end, we performed the time‐of‐addition experiment and revealed that LL‐37 prevented SARS‐CoV‐2 spike pseudovirus entry only if added prior (2 h) to or during infection but not if added 2 h post infection (hpi) and 4 hpi (Figure 2A); LL‐37 showed no inhibition of SARS‐CoV‐2 spike pseudovirus when only pretreated 293T cells that stably express human ACE2 (293T‐A2) (Figure 2B). Here, ACE2 is linked to infection.