At the 95 th percentile threshold of the liver control groups, pronounced hypermethylation was detected in 28 out of 54 non-cirrhotic HCC tissues for FGF19 (51.9%), 21 for NKX2-4 (38.9%), 40 for SPAG6 (74.1%), 38 for FOXD3 (70.4%), 22 for NRIP3 (40.7%), 21 for NKX3-2 (38.9%), 25 for TBX4 (46.3%), 20 for TSPYL5 (37.3%), 16 for GRASP (29.6%), 33 for BOP1 (61.1%) and 23 for C8orf82 (42.6%) (Fig. 3F). This evidence concerns the gene FOXD3 and hepatocellular carcinoma.