At the 95 th percentile threshold of the cirrhosis group, significant hypermethylation was observed in 16 out of 27 cirrhotic HCC tissues for FGF19 (59.3%), 8 for NKX2-4 (29.6%), 12 for SPAG6 (44.4%), 14 for FOXD3 (51.9%), 20 for NRIP3 (74.1%), 10 for NKX3-2 (37.0%), 18 for TBX4 (66.7%), 19 for TSPYL5 (70.4%), 17 for GRASP (63.0%), 25 for BOP1 (92.6%) and 19 for C8orf82 (70.4%) (Fig. 3C). Here, SPAG6 is linked to hepatocellular carcinoma.