CD2 and neoplasm: Similarly, the ElastiGate algorithm captured CD2 downregulation without interfering with the resolution of the Cell Trace Violet+ (CTV+) in proliferating tumor cells, while flowDensity algorithm reverted the horizontal threshold and placed the quad gate (CD2/CTV) on the opposite (incorrect) side of the cluster in some cases, despite the use of optimized input parameters (Supplementary Figure 6).