Moreover, as autophagy is impaired in MJD [7], we observed, as expected, a tendency reduction in the levels of Beclin-1 and p62 in the MJD EVs as compared with CNT EVs, since these proteins are sequestered in protein inclusions in MJD cells [7], with a consequent decrease in the soluble fraction and consequently in the EVs. Here, BECN1 is linked to Machado-Joseph disease.