Through RT-qPCR the autophagy-related SQSTM1, BECN1, UBC, ATG12, and LC3B mRNA were detected and quantified as well as the oxidative stress-related CYCS mRNA levels in CNT and MJD NESC-EVs (Fig. 2F). This evidence concerns the gene BECN1 and Spinocerebellar ataxia type 3.