The results showed that the infiltration levels of CD8+ T cells and tumor-associated macrophages (TAMs) cells were different between the shnc group and shcircGramd4 group, consistent with the single-cell sequencing analysis results in Figure 1D, but there was no significant difference statistically, which may be related to the short growth time of subcutaneous tumors in mice. Here, CD8A is linked to neoplasm.