To address the role of PINLYP during KSHV latent infection, we used the CRISPR/Cas9 gene editing system to generate PINLYP knockout (KO) iSLK.RGB cells, which harbor latent KSHV reporter virus that contains a doxycycline (Dox)-inducible RTA to enable entry into the lytic cycle and marks red fluorescent latent, green fluorescent immediate early, and blue fluorescent late viral gene expression. Here, PINLYP is linked to disease arising from reactivation of latent virus.