The AOM/DSS model resembles the human adenomas of colitis associated-CRC and, in particular, our organoids present a mutation in the exon 3 of ctnnb1 gene (with wild-type Kras, data not shown) leading to an aberrant Wnt pathway activation [34], a signaling pathway whose alteration is fundamental for the initiation and progression of CRC [35]. Here, KRAS is linked to adenoma.