To further support the relevance of deleting TMEM219 in ISCs in an immune-mediated enteritis model, we administered a Toll-like receptor–3 agonist, (Poly I:C), which activates immune response, in the ISC-Tmem219–/– and ISC-B6 mice in a preventive approach using a short-term protocol. This evidence concerns the gene TMEM219 and enteritis.