Study calculated the area under the receiver operating characteristic curves (area under the curve) and found that urinary IgG (0.894), NGAL (0.875), TRF (0.861), TNF-α (0.763), and the combination of urinary TRF + IgG + TNF-α + NGAL (0.922) showed good diagnostic value for early-stage DN. This evidence concerns the gene TNF and liver dysplastic nodule.