To investigate whether these mechanisms are involved in the anti-mycobacterial activity of Vγ9Vδ2 T cells, we respectively analyzed the expression of cytotoxicity-related factors, including granzyme A (GrzA), granzyme B (GrzB), perforin (PRF), and granulysin (GNLY), in cell supernatants collected at 48 h and 72 h post-infection using a CBA assay. The gene discussed is PRF1; the disease is infection.