To confirm the role of PAX7 in breast cancer, we generated stable transfection cell lines with reduced PAX7 expression in MDA‐MB‐468 and MDA‐MB‐231 cells (Figure 5B–E) and conducted high‐throughput sequencing on the MDA‐MB‐231 cell line with PAX7 knockdown (Figure S1 display the quality control of high‐throughput sequencing data). This evidence concerns the gene PAX7 and breast cancer.